Scitech presents the new Luxendo MuVi-SPIM, an advanced single plane
illumination microscope designed to facilitate rapid fluorescence imaging of
biological specimens with sub-cellular resolution.
The MuVi-SPIM utilises a sheet of laser light to illuminate only a thin
slice of a fluorescently labelled sample. A wide-field fluorescence microscope
placed perpendicular to the light sheet collects the fluorescence signal and
images the observed region using an sCMOS camera. Optimised combinations of the
sCMOS camera and objective lenses allow the user to obtain multiple views of
large samples at unprecedented speeds.
MuVi-SPIM is ideal for applications in developmental biology, embryonic
development, marine biology, neurobiology, functional imaging, long time lapse
imaging and large specimens.
By providing four simultaneous orthogonal views on large living
specimens without the need for sample rotation, MuVi-SPIM eliminates shadowing
effects and facilitates long-term imaging at ultra-high acquisition speeds. Considered
the fastest SPIM on the market for analysing dynamic processes, the microscope
enables four simultaneous views at 65 frames per second. Sample photo toxicity is
avoided by sequentially illuminating a stack of small slices of an organism.
MuVi-SPIM integrates modular software concepts in order to enhance
flexibility in designing complex experimental layouts, and 3D image data is
employed in real-time, and directly streamed to a storage and data processing
Key features of Luxendo’s MuVi-SPIM microscopes include large field of
view, close-to-confocal resolution, very high imaging speed, great sample
handling and low ototoxicity, high sensitivity and low noise, 360-degree
illumination and detection, high-speed sCMOS camera Hamamatsu ORCA-Flash 4.0, laser
combiner with six laser positions, 2x Nikon CHI Plan Fluor 10XW 0.3NA water
immersion for illumination, 2x Nikon CFI LWD 25XW 1.1NA water immersion for
detection, and mounting chamber with temperature control (15¡C – 40¡C).
For more information, please visit the Scitech website
www.scitech.com.au or call (03) 9480