Holotomography and 3D fluorescence imaging in one microscope

Scitech presents the HT-2 from Tomocube designed to combine both
holotomography and 3D fluorescence imaging into one microscope.

The HT-2 facilitates three-dimensional fluorescence and optical
diffraction tomography of live cells using structured illumination with minimal
stress on cells. This technology expands the use of the microscope to a variety
of previously unexplored applications in the fields of bioscience and life
science, enabling researchers and clinicians to break into new frontiers of knowledge,
diagnosis and disease treatment.

The HT-2 is able to achieve label-free imaging of cells and allows users
to conduct long-term tracking of specific areas within live cells. It has the
capacity to deliver correlative analysis in 2D, 3D and 4D with HT and
fluorescence images, and incorporates a customisable three-channel LED light
source (385 nm, 470 nm, 570 nm). HT-2 generates Z-stack images with a motorised
Z-drive (step resolution: 150nm).

Benefits of Tomocube HT-2 microscopes:

Correlative microscopy in one
instrument

HT-2 provides high-quality 3D images of both holotomography and 3D
fluorescence for each sample.

Quantitative data marked with
fluorescence

HT-2 provides morphological (volume, surface area, projection area,
sphericity and ellipticity), chemical (dry mass, concentration) and mechanical
(cell deformability) properties of cells with 3D refractive index (RI)
tomogram. The fluorescence image also provides information about molecular
specificity.

Live cell molecular and
holographic imaging with minimal stress on cells

Simultaneous measurement capability of time-lapse 3D RI tomography and
fluorescence image allows long-time tracking of specific targets in live cells.
The fluorescence image provides the position of specific target organelles or
structures in live cell, and consecutive measurements of time-lapse 3D RI
tomography enable the monitoring of cells and their structures with minimal
stress.

For more information, please visit the Scitech website
www.scitech.com.au or call (03) 9480 4999.

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