Scitech’s imaging and illumination solution for in vivo experiments on live animals

Scitech presents the Optical Activation, Stimulation and Imaging System
(OASIS), a new imaging and illumination solution for in vivo optogenetics,
photostimulation and photoactivation experiments on live animals.

Typically configured as a macroscope with high NA objective
magnification options of 1.9x, 2.35x and 4x, the OASIS offers a large field of
view. The macroscope can record and control the activity of each neuron in the
brain of behaving animals at a single cell and ms resolution using the
Polygon400 patterned illuminator to deliver light to a specified area of the
specimen while leaving neighbouring areas untouched.

OASIS allows flexible reconfiguration options, and can be configured to
work around a fixed specimen such as a sedated live animal by rotation and
translation. A heavy-duty precision XYZ stage is mounted on the support
structure and the OASIS optical assembly is mounted on the XYZ stage, which
controls the lateral motion as well as focus of the system. A pan/tilt
mechanism add-on can be used to orient the macroscope relative to the live
animal. Both the XYZ stage and tilt mechanism are available in motorised
versions.

Flexible configuration options for the OASIS also include a microscope
instead of macroscope optical path, allowing for integration of Olympus, Nikon,
Zeiss and Leica infinity-corrected objectives; dual camera ports; multiple
Polygon400 illuminators coupled into the lightpath; and patterned
illumination/widefield combinations.

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