Dual Inverted Selective Plane Illumination Microscopy

Applied Scientific
Instrumentation (ASI) has developed a new form of light sheet microscopy. The Dual
Inverted Selective Plane Illumination Microscopy (diSPIM)
system utilizes two water emersion objectives mounted at 45 degree angles above
the sample on an inverted microscope.

third objective can also be used to view the sample from below. The top
objectives are mounted on piezo positioning devices so precise image stacks can
be obtained. The system utilizes micro-mirrors to generate the light sheet, and
the complete system can be mounted on to an existing inverted microscope, or
supplied with ASI’s Rapid Automated Modular Microscope system (RAMM).


  • Low photobleaching: >10x reduction
    vs. confocal/spinning disk
  • Rapid 3D imaging with isotropic
    resolution (330 nm in all directions)
  • ~2x better axial resolution than
    confocal/spinning disk systems
  • Acquisition rates up to 200 planes per
  • Microscope has multicolor capability
  • Use conventional sample mounting on
    glass cover slips or open dish
  • Tested successfully on cells embedded in
    collagen gels and nematode and zebrafish embryos.

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