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Dual Inverted Selective Plane Illumination Microscopy

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Applied Scientific Instrumentation (ASI) has developed a new form of light sheet microscopy. The Dual Inverted Selective Plane Illumination Microscopy (diSPIM) system utilizes two water emersion objectives mounted at 45 degree angles above the sample on an inverted microscope.

A third objective can also be used to view the sample from below. The top objectives are mounted on piezo positioning devices so precise image stacks can be obtained. The system utilizes micro-mirrors to generate the light sheet, and the complete system can be mounted on to an existing inverted microscope, or supplied with ASI’s Rapid Automated Modular Microscope system (RAMM).

Features:

  • Low photobleaching: >10x reduction vs. confocal/spinning disk
  • Rapid 3D imaging with isotropic resolution (330 nm in all directions)
  • ~2x better axial resolution than confocal/spinning disk systems
  • Acquisition rates up to 200 planes per second
  • Microscope has multicolor capability
  • Use conventional sample mounting on glass cover slips or open dish
  • Tested successfully on cells embedded in collagen gels and nematode and zebrafish embryos.

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