The Automation Partnership, a company specialising in automated life science applications is using OEM pumps from Watson-Marlow Bredel on a fully automated flexible batch cell culture machine.
Headquartered in Royston, Hertfordshire, The Automation Partnership (TAP) has designed the SelecT machine with a consortium of six major pharmaceutical companies to meet the challenge of producing high quality, consistent cells for drug discovery on demand.
This automated cell culture machine uses peristaltic pumps from Watson-Marlow Bredel.
Based on recommendations from members of the consortium, which includes GlaxoSmithKline, Merck, Pfizer, Bristol-Myers Squibb, AstraZeneca and Parke-Davies, TAP contacted Watson-Marlow Bredel for peristaltic pumps that would provide a high performance metering solution with an easy-to-clean design.
Typically measuring around 2m x 2m x 3m, the SelecT machine provides a completely controlled class 100 HEPA-filtered environment for totally sterile cell culture growth.
This allows pharmaceutical companies to grow and harvest cell lines overnight or at weekends without the need for supervision, to enable screening five days a week.
The SelecT cell culture system relies on up to 15 Watson-Marlow 314D peristaltic pumpheads vertically mounted on the machine's external front face, metering process fluids such as cell culture media or PBS rinse solution.
Using silicone tubing linked to a rack or refrigerator containing the fluids, liquid is transferred into the sealed unit of the SelecT for metering onto cells.
The SelecT also employs two Watson Marlow 313FAC pumps externally mounted alongside the 314D pumpheads.
These pumps transfer the fluid required for the washing and priming station to be used in cleaning the cell pooling and dispensing pot. The cells are then dispensed into multiwell plates.
David Jervis, Applications Manager at TAP comments that the SelecT allows entire automation of the process unlike manual cell cultivation methods, resulting in increased consistency and individual cell traceability.
The 313FAC and 314D peristaltic pumps from Watson-Marlow Bredel are specially designed for 24-hour operation.
The 313FAC peristaltic pumps have flow rates at 50Hz up to 1350ml/minute at 270rpm.
The 314D pumps have four rollers for minimum pulsation and accept seven tube sizes without the need for adjustment.