Scitech introduces the MetaMorph super-resolution system from Molecular Devices providing real-time super-resolution imaging on the nanometer scale.
Capable of capturing organisation of sub-cellular structures not readily discernible with conventional confocal or TIRF microscopy, the MetaMorph super-resolution system can be used to resolve sub-cellular objects as small as 20 nm spatially and 40 nm axially. The MetaMorph super-resolution system provides a means to control experimental hardware, capture image sequences, perform localization calculations and display the developing super-resolution image in real-time.
Offering synchronised image acquisition and processing, the MetaMorph super-resolution system enables analysis of object details smaller than 250 nm in fixed and live cells. It features real-time image processing, expanding the capabilities of optical microscopy in research and supports numerous scientific applications from time-lapse studies to 3D investigations.
Light microscopy cannot be relied upon to analyse images of biological matter smaller than 250 nm. Super-resolution microscopy helps to overcome these limits by reconstructing images at a resolution surpassing the diffraction limit of the objective lens. With patent-pending image processing techniques, the system produces 20 nm lateral resolution in real time.
The MetaMorph super-resolution system builds on the MetaMorph software that guides researchers through setup, acquisition and analysis, with a dedicated workspace in the user interface ensuring that relevant configuration and display settings are accessible. Side-by-side displays and image statistics provide a multifaceted view of the quality and progress of image acquisition and processing.
A unique hardware acceleration component supports fast acquisition and analysis with real-time super-resolution image display. An optional off-line mode allows super-resolution processing of previously acquired images. The system is compatible with most fluorescence and TIRF microscopes.
- Supports PALM, STORM, dSTORM super-resolution techniques
- Compatible with most microscopes and TIRF systems
- Live replay captures real time events such as in FRAP, FRET and other laser-based studies, time lapse experiments, live cell imaging, and digital microscopy
- Lateral resolution 20 nm; axial resolution 40 nm
- Supports many single molecule localization super-resolution techniques
- Real-time super-resolution image construction at camera frame rate
- Offline analysis available
- Wavelet filtering and Gaussian fitting
- 3-D localization using astigmatism
- Real-time super-resolution image display at any CCD frame rate
- Offline processing
- Drift correction using fiduciary markers
- Variable scaling of super-resolution image
- Automatic thresholding and splitting of closely spaced molecules
- Single molecule localization text file generation for data exportation
- Image stack acquisition
- Arbitrary acquired image size