Scitech have announced the Hyperspectral imaging technology. The Cytoviva Hyperspectral imaging system (HSI) provides spectral analysis of materials and biologicals imaged with the CytoViva Nano-scale optical microscope. Operating in the visible near infrared spectral range (VNIR), the CytoViva Hyperspectral imaging system supports research in areas such as nano-drug delivery and nano-toxicology. This spectral analysis method supports both non-fluorescent and fluorescently labelled components in live cells and nano-materials.
In each application, the CytoViva Nano-scale optical microscope and Cytoviva Hyperspectral imaging system work together to provide analytical confirmation of nano-scale materials and their interactions with biologicals or composite materials. The Cytoviva Hyperspectral imaging system collects spectra from a samples light scatter. The Cytoviva Hyperspectral imaging system is an alternative to spectroscopy methods as it collects the complete spectra from each individual pixel in the field of view.
The CytoViva Hyperspectral imaging system combines the components of a concentric imaging spectrophotometer that contains original, aberration-corrected convex holographic diffraction gratings. Operating in the 400 to 1,000 nm spectral range, the concentric imaging spectrophotometer technology provides higher signal-to-noise ratio insuring that only spectra from the high contrast CytoViva Nano-scale optical microscope image is captured for analysis.
The CytoViva Hyperspectral imaging system combines a customised hyperspectral image analysis software designed to support microscopy applications. This proprietary software is a modified version of the ITT Visual Information Solutions ENVI hyperspectral image analysis system. As a data collection and reporting capability, the hyperspectral image analysis software is easy to use and intuitive. Using this system, the spectral analysis can be performed within a user defined region of interest down to the pixel level. The hyperspectral image analysis software enables the researcher to locate regions within the sample that matches qualified spectra that has been preloaded in a library. It also provides the ability to un-mix spectra to isolate specific sample components or sample anomalies for further evaluation.
The CytoViva Hyperspectral imaging system goes through a motorised stage and is integrated onto the CytoViva microscope system. The CytoViva Hyperspectral imaging system acts as a ‘push broom’, moving the sample precisely over the hyperspectral image detector field of view. Operation of this push broom capability is integrated into the image analysis software system.
The CytoViva Hyperspectral imaging system is used in the following applications:
- Nanoscale studies
- Nano-drug delivery
- Nano particles used as bio-markers
- Nano-toxicology (measuring the presence of nano-materials in cells and tissues)
- Nano-materials analysis (confirmation of different elements within composites and coatings on materials)
- Identification of pathogens and biological agents