The Institute for Radiopharmacy at the Forschungszentrum Dresden-Rossendorf in Germany is using NanoSight’s LM20 nanoparticle characterisation system to study magnetic nanoparticles for applications in cancer therapy.
The research team of Dr Holger Stephan is working to develop magnetic nanoparticles, which can be applied in cancer therapy preferably in combination with intracellular hyperthermia/ ablation and endoradionuclide therapies.
This requires nanoparticles to be very stable under physiological conditions, also avoiding accumulation in the reticuloendothelial system.
Many techniques had been used to characterise the nanoparticles before Dr Stephan discovered the NanoSight system. These included Photon Correlation Spectroscopy (PCS), scanning electron microscopy (SEM) and ultra centrifugation.
The NanoSight ability to track individual particles by the scattering of a laser beam has made the study of nanoparticle stability much more convenient. Nanoparticle Tracking Analysis (NTA) has been used to successfully follow the stability of different systems as a function of temperature in EDTA and different cell media.
There are three specific goals for measurement:
- Get information about the size distribution of nanoparticles and their stability under physiologically relevant conditions
- Study the influence of nanoparticle surface modification on the size distribution
- Achieve structure-activity relationships for the nanoparticles on the cellular uptake behaviour and cancer tissue accumulation
Dr Stephan has described several advantages of the NanoSight LM20 system. These include:
- Reliable results on nanoparticles as small as 50nm
- Straightforward sample handling and measurement as well as quicker experiments
- Ability to work directly with relevant solutions facilitates quick understanding of the behaviour of these magnetic nanoparticles and their ultimate use in treating cancers
This characterisation information is highly informative in understanding the more complex suspensions in biological systems and has led to its wide application in development of drug delivery systems, viral vaccines, study of toxicology of nanoparticles and their environmental fate as well as biomarker detection.
This real-time data also provides insight into the kinetics of protein aggregation and other time-dependent phenomena in a quantitative manner at deeply sub-micron sizes.